Cell death induced by ozone and various non-thermal plasmas: therapeutic perspectives and limitations
نویسندگان
چکیده
Non-thermal plasma has been recognized as a promising tool across a vast variety of biomedical applications, with the potential to create novel therapeutic methods. However, the understanding of the molecular mechanisms behind non-thermal plasma cellular effects remains a significant challenge. In this study, we show how two types of different non-thermal plasmas induce cell death in mammalian cell cultures via the formation of multiple intracellular reactive oxygen/nitrogen species. Our results showed a discrepancy in the superoxide accumulation and lysosomal activity in response to air and helium plasma, suggesting that triggered signalling cascades might be grossly different between different plasmas. In addition, the effects of ozone, a considerable component of non-thermal plasma, have been simultaneously evaluated and have revealed much faster and higher cytotoxic effects. Our findings offer novel insight into plasma-induced cellular responses, and provide a basis for better controlled biomedical applications.
منابع مشابه
Effect of Three Therapeutic Methods of Exercise, Ozone, and Stem Cells on the MEF2C Expression and Myostatin Levels in Femoral Muscle Tissue of the Osteoarthritis Rats
Aims Myostatin and Myocyte Enhancer Factor 2C (MEF2C) are involved in muscle changes associated with bone problems. The aim of the present study was to determine the effect of three therapeutic methods of exercise, ozone, and stem cells on MEF-2C gene expression and myostatin levels of femoral muscle tissue in osteoarthritis rats. Methods & Materials This experimental study was done on 63 male...
متن کاملModulational instability of dust ion acoustic waves in astrophysical dusty plasmas with non thermal electrons
Propagation of dust ion acoustic waves in plasmas composed of nonthermal distributed electrons and stationary dust particles is investigated. Nonlinear Schrdinger equation is derived to describe small amplitude waves, using the reduction perturbation technique. Modulation instability of dust ion acoustic waves is analysed for this system. Parametric investigation indicates that growth rate of...
متن کاملCell damaging by irradiating non-thermal plasma to the water: Mathematical modeling of chemical processes
Recently non-thermal plasma (NTP) is applied for many therapeutic applications. By NTP irradiating to the tissues or cell-lines, the water molecules (H2O) would be also activated leading to generate hydrogen peroxide (H2O2). By irradiating plasma to bio-solution, its main output including vacuum UV to UV causes the photolysis of H2O leading to generat...
متن کاملKinetics of cell death triggered photothermally using folate-conjugated gold nanoparticles and various laser irradiation conditions in cancer cells
Introduction: In this study, we explore in detail cell-specific targeting efficacy of nano-photo-thermal therapy (NPTT) method and the resulting responses that are induced by variable laser intensities and exposure times in cancer cells to induce selective apoptosis. We delineate the synthesis of a high-yielding synthetic F-AuNPs by tailoring the surface of gold nanoparticles ...
متن کاملCell Death Induced on Cell Cultures and Nude Mouse Skin by Non-Thermal, Nanosecond-Pulsed Generated Plasma
Non-thermal plasmas are gaseous mixtures of molecules, radicals, and excited species with a small proportion of ions and energetic electrons. Non-thermal plasmas can be generated with any high electro-magnetic field. We studied here the pathological effects, and in particular cell death, induced by nanosecond-pulsed high voltage generated plasmas homogeneously applied on cell cultures and nude ...
متن کامل